bloodjourГематология и трансфузиологияRussian journal of hematology and transfusiology0234-57302411-3042ООО Издательский дом «Практика»10.18821/0234-5730-2016-61-3-133-137bloodjour-19Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВосстановление CD4+ Т-клеток, экспрессирующих молекулу CD31, у больных лимфопролиферативными заболеваниями после трансплантации гемопоэтических стволовых клетокRecovery of CD4+ CD31+ T-cell in patients with lymphoproliferative disorders following hematopoietic stem cell transplantationhttps://orcid.org/0000-0003-2902-9336БаторовЕ. В.BatorovE. V.

Баторов Егор Васильевич, кандидат мед. наук, научный сотрудник лаборатории клеточной иммунотерапии 

Scopus Author ID 35768879800

630099, г. Новосибирск

Batorov Egor V., MD, PhD, researcher of the Laboratory of Cellular Immunotherapy

Scopus Author ID 35768879800

Novosibirsk, 630090

Ebatorov@mail.ruТихоноваМ. А.TikhonovaM. A.

630099, г. Новосибирск

Novosibirsk, 630090

КрючковаИ. В.KryuchkovaI. V.

630099, г. Новосибирск

Novosibirsk, 630090

СергеевичеваВ. В.SergeevichevaV. V.

630099, г. Новосибирск

Novosibirsk, 630090

СизиковаС. А.SizikovaS. A.

630099, г. Новосибирск

Novosibirsk, 630090

БатороваД. С.BatorovaD. S.

630099, г. Новосибирск

Novosibirsk, 630090

УшаковаГ. Ю.UshakovaG. Yu.

630099, г. Новосибирск

Novosibirsk, 630090

ГилевичА. В.GilevichA. V.

630099, г. Новосибирск

Novosibirsk, 630090

ОстанинА. А.OstaninA. A.

630099, г. Новосибирск

Novosibirsk, 630090

https://orcid.org/0000-0003-2346-6279ЧерныхЕ. Р.ChernykhE. R.

Researcher ID: K-1052-2014

630099, г. Новосибирск

Researcher ID: K-1052-2014

Novosibirsk, 630090

ФГБНУ «Научно-исследовательский институт фундаментальной и клинической иммунологии»РоссияResearch Institute of Fundamental and Clinical ImmunologyRussian Federation201626022019613133137Copyright © Баторов Е.В., Тихонова М.А., Крючкова И.В., Сергеевичева В.В., Сизикова С.А., Баторова Д.С., Ушакова Г.Ю., Гилевич А.В., Останин А.А., Черных Е.Р., 20192019Баторов Е.В., Тихонова М.А., Крючкова И.В., Сергеевичева В.В., Сизикова С.А., Баторова Д.С., Ушакова Г.Ю., Гилевич А.В., Останин А.А., Черных Е.Р.Batorov E.V., Tikhonova M.A., Kryuchkova I.V., Sergeevicheva V.V., Sizikova S.A., Batorova D.S., Ushakova G.Y., Gilevich A.V., Ostanin A.A., Chernykh E.R.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.htjournal.ru/jour/article/view/19

Цель работы – исследование динамики восстановления CD4+CD45RA+CD31+ Т-клеток и ранее не описанной субпопуляции CD4+CD45RA-CD31+ Т-клеток у больных лимфопролиферативными заболеваниями после высокодозной химиотерапии с трансплантацией аутологичных гемопоэтических стволовых клеток (ауто-ТГСК). В исследование вошли 87 больных. Методом проточной цитометрии оценивали количество CD4+CD45RA+CD31+ и CD4+CD45RA-CD31+ Т-клеток перед ауто-ТГСК, на день выхода из лейкопении, через 6 и 12 мес после ТГСК. Относительное количество CD4+CD45RA-CD31+ T-клеток значимо повышено у больных по сравнению со здоровыми лицами и восстанавливается после ауто-ТГСК до исходных показателей ко дню выхода из лейкопении. Посттрансплантационная лучевая терапия на область средостения приводит к значимому снижению количества CD4+CD45RA+CD31+ Т-клеток и удлиняет период их восстановления. Облучение мягких тканей не приводит к значимому снижению этой субпопуляции клеток. При изучении восстановления CD4+CD45RA+CD31+ Т-клеток методом проточной цитометрии нужно исключать из исследования CD31+ T-клетки памяти.

We have evaluated the dynamics of post-transplant recovery of CD4+CD45RA+CD31+ T cells and CD4+CD45RACD31+ T-cells in patients with lymphoproliferative diseases after high-dose chemotherapy with autologous stem cell transplantation (auto-HSCT). 87 patients were included in the study. The content of circulating CD4+СD31+ naïve and memory T-cells has been assessed with the use of flow cytometry before auto-HSCT, at the day of engraftment, and in 6 and 12 months. Relative amount of CD4+CD45RACD31+ T-cells in patients was elevated in comparison with healthy controls, restored rapidly following auto-HSCT and reached initially high level at the day of engraftment. Post-transplant mediastinal radiotherapy significantly reduced counts of CD4+CD45RA+CD31+ T-cells and extended recovery period compared to the non-irradiated patient level. Non-thymic tissue irradiation reduced this subset slightly and non-significantly. The study of the recovery of CD4+CD45RA+CD31+ T-cells by virtue of flow cytometry required an accurate gating strategy to exclude CD31+ T memory cells.

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The authors declare that there are no conflicts of interest present.