bloodjourГематология и трансфузиологияRussian journal of hematology and transfusiology0234-57302411-3042ООО Издательский дом «Практика»10.35754/0234-5730-2020-65-3-281-290bloodjour-231Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПродукция биопленок среди возбудителей инвазивного кандидоза у больных опухолевыми заболеваниями системы крови и у больных без опухолевых заболеваний системы кровиBiofi lm production among Candida spp. causing invasive candidiasis in patients with hematological malignancies and without hematological malignancieshttps://orcid.org/0000-0002-8725-5131МальчиковаА. O.MalchikovaA. O.

Мальчикова Анна Олеговна, кандидат медицинских наук, научный сотрудник лаборатории клинической бактериологии, микологии и антибиотической терапии

Anna O. Malchikova, Сand. Sci. (Med.), Researcher, Laboratory of Clinical Microbiology, Mycology and Antibiotic Therapy

anmalchikova@gmail.comhttps://orcid.org/0000-0001-5973-5763КлясоваГ. А.KlyasovaG. A.

Клясова Галина Александровна, доктор медицинских наук, профессор, заведующая лабораторией клинической бактериологии, микологии и антибиотической терапии

Galina A. Klyasova, Dr. Sci. (Med.), Prof., Head of the Laboratory of Clinical Microbiology, Mycology and Antibiotic Therapy

klyasova.g@blood.ruФГБУ «Национальный медицинский исследовательский центр гематологии» Министерства здравоохранения Российской ФедерацииРоссияNational Research Center for HematologyRussian Federation202020092020653281290Copyright © Мальчикова А.O., Клясова Г.А., 20202020Мальчикова А.O., Клясова Г.А.Malchikova A.O., Klyasova G.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.htjournal.ru/jour/article/view/231Введение

Введение. Негативное влияние на  результаты лечения инвазивного кандидоза может оказывать способность Candida spp. формировать биопленки на инвазивных медицинских устройствах у разных категорий больных.

Цель

Цель: оценить способность образовывать биопленки разных видов Candida, выделенных из клинически значимых образцов от больных опухолями системы крови и больных без опухолей системы крови.

Материалы и  методы

Материалы и  методы. Определение способности Candida spp. продуцировать биопленки проводили спектрофотометрическим методом с  использованием соли тетразолия (XTT, Sigma-Aldrich, США). Изоляты Candida spp., имеющие значение оптической плотности от  0,1  и  более, оценивали как образующие биопленки, менее 0,1 — как не способные к формированию биопленок.

Результаты

Результаты. Было исследовано 428 Candida spp. (C. albicans n = 192, C. parapsilosis complex n = 121, C. krusei n = 40, C. tropicalis n = 38 и C. glabrata n = 37), из них 172 изолята были выделены от больных опухолями системы крови, 256 — от больных без опухолей системы крови, 361 — из гемокультуры, 67 — из других стерильных образцов. Способность формировать биопленки была определена у  41,8 % (n = 179) Candida spp., с  одинаковой частотой у  больных опухолями системы крови и больных без опухолей системы крови (41,9 и 41,8 % соответственно). Candida non-albicans чаще продуцировали биопленки в сравнении с C. albicans (52,5 % против 28,6 %, соответственно, р < 0,001). Значимо чаще продукция биопленок была у C. tropicalis (89, 5 %) и у C. krusei (75 %) в сравнении с C. parapsilosis (41,3 %), C. albicans (28,6 %) и C. glabrata (27 %, p < 0,05). Частота образования биопленок была высокой для C. krusei и C. tropicalis в обеих группах больных, в то время как C. albicans, выделенные от больных без опухолей системы крови, достоверно чаще продуцировали биопленки, чем в группе сравнения (34,1 % против 18 % соответственно, p = 0,03). Продукция биопленок была сопоставимой среди Candida spp., выделенных из  гемокультуры (42,9 %) и из других стерильных образцов (35,8 %, р = 0,3).

Заключение

Заключение. Способность к  формированию биопленок различалась у  разных видов Candida и  преобладала у C. tropicalis и C. krusei. Продукция биопленок была выявлена с одинаковой частотой у больных опухолями и без опухолей системы крови.

Introduction

Introduction. Biofi lm-forming ability among Candida spp. on indwelling medical devices may have a negative infl uence on the outcome of invasive candidiasis in various groups of patients.

Aim

Aim. The objective of this study was to evaluate the biofi lm-forming ability among Candida spp. isolated from clinical specimens in patients with hematological malignancies and patients without hematological malignancies.

Materials and methods

Materials and methods. Biofi lm production among Candida spp. was studied using XTT (Sigma-Aldrich, USA) reduction assay. Candida spp. were classifi ed as biofi lm-forming, having optical density equal to and more than 0.1, and non-biofi lmforming with optical density less than 0.1.

Results

Results. A total of 428 Candida spp. (C. albicans n = 192, C. parapsilosis n = 121, C. krusei n = 40, C. tropicalis n = 38, C. glabrata n = 37) were evaluated (172 from hematological patients, 256 from non-hematological patients, 361 from blood culture, 67 from other sterile specimens). Biofi lm-forming ability was detected among 179 (41.8%) Candida spp. with the same rate in hematological patients and non-hematological patients (41.9 % and 41.8 %, respectively). Biofi lm production predominated among non-C. albicans (52.5 %) compared to C. albicans (28.6 %, p = 0.001). Biofi lm production prevailed among C. tropicalis (89.5 %) and C. krusei (75 %) compared to C. parapsilosis (41.3 %), C. albicans (28.6 %), and C. glabrata (27 %, respectively, p < 0.05). Biofi lm-forming ability among C. tropicalis and C. krusei dominated in both groups of patients. Biofi lm production among C. albicans prevailed in non-hematological patients compared to hematological patients (34.1% vs 18.2%, p = 0.03). There were no differences in biofi lm production among Candida spp. isolated from blood culture (42.9%) and other sterile specimens (35.8%, p = 0.3).

Conclusion

Conclusion. Biofi lm-forming ability varied among the Candida spp. and prevailed among C. tropicalis and C. krusei. Biofi lm production among Candida spp. was detected with the same rate in hematological and non-hematological patients.

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The authors declare that there are no conflicts of interest present.