bloodjourГематология и трансфузиологияRussian journal of hematology and transfusiology0234-57302411-3042ООО Издательский дом «Практика»10.35754/0234-5730-2021-66-3-386-394bloodjour-299Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВлияние криоконсервирования на показатели апоптоза мононуклеаров при проведении экстракорпорального фотоферезаThe effect of cryopreservation on the parameters of mononuclear apoptosis during extracorporeal photopheresishttps://orcid.org/0000-0003-0904-7385ВасильеваВ. А.VasilyevaV. A.

Васильева Вера Алексеевна,* кандидат медицинских наук, заведующая отделением иммунохимиотерапии для больных после ТКМ и группой поиска потенциальных доноров

 125167, Москва 

 Vera A. Vasilyeva,* Cand. Sci. (Med.), Head of the Department of Immunochemotherapy for Patients after BMT 

 125167, Moscow 

vasilievava4@mail.ruhttps://orcid.org/0000-0001-6201-6276КузьминаЛ. А.KuzminaL. A.

Кузьмина Лариса Анатольевна, кандидат медицинских наук, заведующая отделением интенсивной высокодозной химиотерапии и трансплантации костного мозга

 125167, Москва 

 Larisa A. Kuzmina, Cand. Sci. (Med.), Head of the Department of Intensive Chemotherapy and BMT 

 125167, Moscow 

kuzlara@rambler.ruhttps://orcid.org/0000-0001-6591-3183ПетинатиН. А.PetinatiN. A.

Петинати Наталия Арнольдовна, кандидат медицинских наук, старший научный сотрудник лаборатории физиологии кроветворения

 125167, Москва 

 Nataliya A. Petinati, Cand. Sci. (Med.), Senior Researcher, Laboratoryfor Physiology of Hematopoiesis 

 125167, Moscow 

loel@mail.ruhttps://orcid.org/0000-0002-7150-0403ДризеН. И.DrizeN. I.

 Дризе Нина Иосифовна, доктор биологических наук, заведующая лабораторией физиологии кроветворения

 125167, Москва 

 Nina I. Drize, Dr. Sci. (Biol.), Head of the Laboratory for Physiology of Hematopoiesis 

 125167, Moscow 

ndrize@yandex.ruhttps://orcid.org/0000-0002-1118-6969КамельскихД. В.KamelskikhD. V.

 Камельских Денис Владимирович, врач-трансфузиолог отделения переливания крови

 125167, Москва 

 Denis V. Kamelskikh, Physician, Department of Blood Transfusion 

 125167, Moscow 

kamelskih@yandex.ruhttps://orcid.org/0000-0002-9684-5045ГапоноваТ. В.GaponovaT. V.

 Гапонова Татьяна Владимировна, кандидат медицинских наук, первый заместитель генерального директора

 125167, Москва 

 Tatyana V. Gaponova, Cand. Sci. (Med.), First Deputy CEO 

 125167, Moscow 

gaponova.tatj@yandex.ruhttps://orcid.org/0000-0002-4119-7175НикифороваК. А.NikiforovaK. A.

Никифорова Ксения Александровна, биолог, лаборатория иммунофенотипирования клеток крови и костного мозга

 125167, Москва 

 Kseniia A. Nikiforova, Biologist, Laboratory of Immunophenotyping of Blood Cells and Bone Marrow 

 125167, Moscow 

nikiforovaksenya@gmail.comhttps://orcid.org/0000-0001-5932-0285ДавыдоваЮ. О.DavidovaYu. O.

 Давыдова Юлия Олеговна, кандидат медицинских наук, врач клинической лабораторной диагностики лаборатории иммунофенотипирования клеток крови и костного мозга

 125167, Москва 

 Yulia O. Davydova, Cand. Sci. (Med.), Laboratory Doctor, Laboratory of Immunophenotyping of Blood Cells and Bone Marrow 

 125167, Moscow 

davydova.y@blood.ruhttps://orcid.org/0000-0002-6512-910XКапрановН. М.KapranovN. M.

Капранов Николай Михайлович, кандидат биологических наук, медицинский физик лаборатории иммунофенотипирования клеток крови и костного мозга

 125167, Москва 

 Nikolay M. Kapranov, Cand. Sci. (Biol.), Medical Physicist, Laboratory of Immunophenotyping of Blood Cells and Bone Marrow 

 125167, Moscow 

kapranov.n@blood.ruhttps://orcid.org/0000-0002-8490-6066ГальцеваИ. В.GaltcevaI. V.

Гальцева Ирина Владимировна, кандидат медицинских наук, заведующий лабораторией иммунофенотипирования клеток крови и костного мозга

 125167, Москва 

 Irina V. Galtseva, Cand. Sci. (Med.), Head of the Laboratory of Immunophenotyping of Blood Cells and Bone Marrow 

 125167, Moscow 

irinagaltseva@gmail.comhttps://orcid.org/0000-0001-6177-3566ПаровичниковаЕ. Н.ParovichnikovaE. N.

 Паровичникова Елена Николаевна, доктор медицинских наук, заведующая отделом химиотерапии гемобластозов, депрессий кроветворения и ТКМ, и. о. генерального директора

 125167, Москва 

 Elena N. Parovichnikova, Dr. Sci. (Med.), Head of the Department of Chemotherapy Hemoblastosis, Hematopoiesis Depression and BMT, acting CEO 

 125167, Moscow 

elenap@blood.ruhttps://orcid.org/0000-0001-8188-5557СавченкоВ. Г.SavchenkoV. G.

 Савченко Валерий Григорьевич , доктор медицинских наук, профессор, академик РАН, генеральный директор

 125167, Москва 

 Valery G. Savchenko , Dr. Sci. (Med.), Full Member of the Russian Academy of Sciences, Director 

 125167, Moscow 

director@blood.ruФГБУ «Национальный медицинский исследовательский центр гематологии» Министерства здравоохранения Российской ФедерацииРоссияNational Research Center for HematologyRussian Federation202123102021663386394Copyright © Васильева В.А., Кузьмина Л.А., Петинати Н.А., Дризе Н.И., Камельских Д.В., Гапонова Т.В., Никифорова К.А., Давыдова Ю.О., Капранов Н.М., Гальцева И.В., Паровичникова Е.Н., Савченко В.Г., 20212021Васильева В.А., Кузьмина Л.А., Петинати Н.А., Дризе Н.И., Камельских Д.В., Гапонова Т.В., Никифорова К.А., Давыдова Ю.О., Капранов Н.М., Гальцева И.В., Паровичникова Е.Н., Савченко В.Г.Vasilyeva V.A., Kuzmina L.A., Petinati N.A., Drize N.I., Kamelskikh D.V., Gaponova T.V., Nikiforova K.A., Davidova Y.O., Kapranov N.M., Galtceva I.V., Parovichnikova E.N., Savchenko V.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.htjournal.ru/jour/article/view/299Введение

Введение. Механизм действия экстракорпорального фотофереза (ЭКФ) связан с индукцией апоптоза лимфатических клеток, которые вызывают активацию антигенпрезентирующих клеток (АПК). В результате применения ЭКФ повышается количество Т-регуляторных лимфоцитов, что индуцирует иммуносупрессивный эффект. Актуальной является проблема использования криоконсервирования мононуклеаров, подвергшихся фотооблучению.

Цель — анализ функциональных особенностей криоконсервированных после ЭКФ мононуклеаров.

Материалы и методы

Материалы и методы. В исследовании проанализированы показатели ранней и поздней стадии апоптоза в концентрате мононуклеаров разных групп в зависимости от времени забора и типа воздействия на данные концентраты мононуклеаров: непосредственно после афереза (группы 1.1 и 1.2), и после ЭКФ (группы 1.3, 2.1 и 2.2), группы 1.2 и 2.2 подвергались криоконсервированию, а 1.3 — криоконсервированию с последующим проведением ЭКФ.

Суммарно проанализировано 113 образцов концентратов мононуклеаров, полученных у 12 больных с хронической реакцией «трансплантат против хозяина» (РТПХ). Все группы образцов были культивированы для определения динамики изменения показателей ранней и поздней стадии апоптоза с течением времени.

Результаты

Результаты. Доля лимфоцитов в поздней стадии апоптоза через двое суток культивирования после стандартной процедуры ЭКФ была сопоставима с долей лимфоцитов в поздней стадии апоптоза при проведении ЭКФ с последующим криоконсервированием лейкоконцентрата.

Заключение

Заключение. Целесообразно проведение сбора мононуклеаров, выполнение их экстракорпорального фотооблучения и в дальнейшем разделение фотооблученных мононуклеаров на несколько частей как для криоконсервирования, так и для возврата некриоконсервированных фотооблученных мононуклеаров больному.

Introduction

Introduction. The mechanism of action of extracorporeal photopheresis (ECP) is associated with the induction of apoptosis of lymphocytes, which cause the activation of antigen-presenting cells (APC). As a result of the use of ECF, the number of T-regulatory lymphocytes increases, which induces an immunosuppressive effect. The actual problem lies in the use of cryopreserved and photo-irradiated mononuclear cells.

Aim — to analyze the functional features of cryopreserved mononuclear cells after ECP.

Materials and methods

Materials and methods. The study analyzed the indicators of early and late stages of apoptosis in the concentrate of mononuclear cells of different groups, depending on the time of collection and the type of exposure on these concentrates of mononuclear cells: immediately after apheresis (groups 1.1 and 1.2), and after ECP (groups 1.3, 2.1 and 2.2), groups 1.2 and 2.2 were after cryopreservation, and 1.3 — after cryopreservation, and then after ECP. 113 samples of mononuclear cell concentrates obtained from 12 patients with chronic graft-versus-host reaction (GVHD) were analyzed. All groups of samples were cultured to determine the dynamics of changes in indicators of early and late stages of apoptosis over time.

Results

Results. The proportion of lymphocytes in the late stage of apoptosis after 2 days cultivation, after the standard ECP procedure, was comparable to the proportion of lymphocytes in the late stage of apoptosis during ECP followed by cryopreservation of the leukoconcentrate.

Conclusion

Conclusion. It is advisable to collect mononuclear cells, perform their extracorporeal photo-irradiation, and then divide the photo-irradiated mononuclear cells into several parts both for cryopreservation and for the return of non-cryopreserved photo-irradiated mononuclear cells to the patient.

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The authors declare that there are no conflicts of interest present.