bloodjourГематология и трансфузиологияRussian journal of hematology and transfusiology0234-57302411-3042ООО Издательский дом «Практика»10.35754/0234-5730-2021-66-4-593-609bloodjour-324Research ArticleОБЗОРЫ ЛИТЕРАТУРЫREVIEWS OF LITERATUREТермолабильность фактора VIII в донорской свежезамороженной плазме кровиThermolability of factor VIII in donor fresh frozen blood plasmahttps://orcid.org/0000-0002-6796-4037ЛемонджаваВ. Н.LemondzhavaV. N.

Лемонджава Вахтанг Нодарович, научный сотрудник отдела исследований и разработки высокоточной медицинской техники 

115201, Москва

Vakhtang N. Lemondzhava, Researcher, Department of Research and Development of High-Precision Medical Equipment 

115201, Moscow

lemonjava.vahtang@gmail.comhttps://orcid.org/0000-0002-7569-0697ЧечеткинА. В.ChechetkinA. V.

Чечеткин Александр Викторович, доктор медицинских наук, директор 

191024, Санкт-Петербург

Alexandr V. Chechetkin, Dr. Sci. (Med.), Director 

191024, St. Petersburg

aschech@rambler.ruhttps://orcid.org/0000-0002-8326-1542ГудковА. Г.GudkovA. G.

Гудков Александр Григорьевич, доктор технических наук, профессор, кафедра РЛ-6 «Технология приборостроения» 

105005, Москва

Alexander G. Gudkov, Dr. Sci. (Tech.), Professor, Department of RL-6 «Instrument Engineering Technology» 

105005, Moscow

profgudkov@gmail.comhttps://orcid.org/0000-0001-7092-360XЛеушинВ. Ю.LeushinV. Yu.

Леушин Виталий Юрьевич, кандидат технических наук, заместитель генерального директора 

115201, Москва

Vitaly Yu. Leushin, Cand. Sci. (Tech.), Deputy General Director 

115201, Moscow

RA3BU@yandex.ruhttps://orcid.org/0000-0002-3597-664XКасьяновА. Д.KasianovA. D.

Касьянов Андрей Дмитриевич, кандидат медицинских наук, руководитель группы контроля качества 

191024, Санкт-Петербург

Andrei D. Kasianov, Cand. Sci. (Med.), Head of the Control Group 

191024, St. Petersburg

kaslab52@mail.ruhttps://orcid.org/0000-0003-1859-3214КиселеваЕ. А.KiselevaE. A.

Киселева Елена Анатольевна, руководитель Республиканского центра гравитационной хирургии крови 

191024, Санкт-Петербург

Elena A. Kiseleva, Head of the Republican Center for Gravity Blood Surgery 

191024, St. Petersburg

kiseleva.rcg@gmail.comООО «Научно-производственная инновационная фирма «ГИПЕРИОН»РоссияHyperion LtdRussian FederationФГБУ «Российский научно-исследовательский институт гематологии и трансфузиологии ФМБА России»РоссияRussian Research Institute of Hematology and TransfusiologyRussian FederationФГБОУ ВО «Московский государственный технический университет им. Н.Э. Баумана (национальный исследовательский университет)»РоссияBauman Moscow State Technical University (National Research University of Technology)Russian Federation202101122021664593609Copyright © Лемонджава В.Н., Чечеткин А.В., Гудков А.Г., Леушин В.Ю., Касьянов А.Д., Киселева Е.А., 20212021Лемонджава В.Н., Чечеткин А.В., Гудков А.Г., Леушин В.Ю., Касьянов А.Д., Киселева Е.А.Lemondzhava V.N., Chechetkin A.V., Gudkov A.G., Leushin V.Y., Kasianov A.D., Kiseleva E.A.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.htjournal.ru/jour/article/view/324Введение

Введение. Критерием показателя качества свежезамороженной плазмы (СЗП) крови является активность в  ней фактора свертывания крови VIII (FVIII).

Цель обзора  — определить технологические барьеры при исследовании термолабильности FVIII и  описать требования к экспериментам, обеспечивающим получение данных о термолабильности FVIII.

Основные сведения

Основные сведения. Проведен анализ публикаций, посвященных исследованию механизмов снижения значения активности FVIII в  донорской плазме крови от  момента донации до  трансфузии. Приведены данные о  снижении активности FVIII на  различных этапах работы с  плазмой крови от  момента донации до  трансфузии. Выполнен анализ методов подготовки образцов для исследования изменения значений FVIII в  донорской плазме крови. Установлено существование противоречивых выводов о влиянии на изменение активности FVIII на этапе оттаивания различных величин воздействий на СЗП крови и малая изученность изменения показателя на этапе подогревания до температуры трансфузии после окончания фазового перехода в образцах. Определены принципиальные отличия в методах подготовки и проведения экспериментов в предшествующих работах. Предложены способы повышения достоверности результатов экспериментов для исследования термолабильности FVIII.

A criterion of the quality of fresh frozen blood plasma (FFP) is the activity of clotting factor VIII (FVIII).

Aim — to identify technological barriers in the study of FVIII thermolability and to describe the requirements for experiments, providing new knowledge about the thermolability of this factor.

Basic information. An analysis of domestic and foreign publications devoted to the study of the mechanisms responsible for reducing the value of FVIII activity in donor blood plasma from the moment of donation to the moment of transfusion was carried out. Data on the decrease in FVIII activity at various stages of work with blood plasma are presented. An analysis of methods for preparing samples for studying changes in the values of FVIII in donor blood plasma was performed. The existence of contradictory conclusions about the infl uence on the change in FVIII at the thawing stage of various values of the effects on FFP and poor knowledge of the change in the indicator at the stage of heating to the transfusion temperature after the end of the phase transition in the samples was established. The fundamental differences in the methods of preparing and conducting experiments in previous works are determined. Methods for increasing the reliability of experimental results for studying the thermal lability of FVIII are proposed.

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The authors declare that there are no conflicts of interest present.