bloodjourГематология и трансфузиологияRussian journal of hematology and transfusiology0234-57302411-3042ООО Издательский дом «Практика»10.35754/0234-5730-2025-70-2-208-228bloodjour-637Research ArticleОБЗОРЫREVIEWSВлияние различных характеристик клеточного продукта на эффективность анти-CD19 терапии Т-лимфоцитами, модифицированными химерным антигенным рецепторомThe Impact of Various Cellular Product Characteristics on the Efficacy of Anti-CD19 Therapy with T-Lymphocytes Modified with Chimeric Antigen Receptorhttps://orcid.org/0009-0004-3139-0176СердюкА. И.SerdyukA. I.

Сердюк Анна Игоревна - лаборант лаборатории трансляционной иммунологии.

125167, Москва

Anna I. Serdyuk - Laboratory assistant, Laboratory of Translational Immunology.

125167, Moscow

anne.serdyuk@gmail.comhttps://orcid.org/0000-0002-4725-6391ИвановаН. О.IvanovaN. O.

Иванова Наталия Олеговна - молекулярный биолог лаборатории трансляционной иммунологии.

125167, Москва

Natalia O. Ivanova - Molecular biologist, Laboratory of Translational Immunology.

125167, Moscow

ivanova.n@blood.ruhttps://orcid.org/0000-0002-9969-8482АлешинаО. А.AleshinaO. A.

Алешина Ольга Александровна - кандидат медицинских наук, заведующая отделом клеточной и иммунной терапии, гематолог отделения гематологии и химиотерапии острых лейкозов и лимфом.

125167, Москва

Olga A. Aleshina - Cand Sci (Med), Head of the Laboratory of cell and immune therapy, Hematologist, Department of Hematology and Chemotherapy of Acute Leukemia and Lymphomas.

125167, Moscow

dr.gavrilina@mail.ruhttps://orcid.org/0000-0002-2687-8482ДиановД. В.DianovD. V.

Дианов Дмитрий Витальевич - стажер-исследователь лаборатории трансляционной иммунологии.

125167, Москва

Dmitry V. Dianov - Researcher, Laboratory of Translational Immunology.

125167, Moscow

dvdianov@gmail.comhttps://orcid.org/0000-0003-0404-1531КузнецоваВ. С.KuznetsovaV. S.

Кузнецова Варвара Сергеевна - лаборант лаборатории трансляционной иммунологии.

125167, Москва

Varvara S. Kuznetsova - Laboratory assistant, Laboratory of Translational Immunology.

125167, Moscow

barbarakuznetsowa@gmail.comhttps://orcid.org/0000-0003-4765-6501МохаммадА.MohammadA.

Мохаммад Афраа - стажер-исследователь лаборатории трансляционной иммунологии.

125167, Москва

Afraa Mohammad - Researcher, Laboratory of Translational Immunology.

125167, Moscow

mohammad.a@blood.ruhttps://orcid.org/0000-0002-8664-6341БоголюбоваА. В.BogolyubovaA. V.

Боголюбова Аполлинария Васильевна - кандидат биологических наук, заведующая лабораторией трансляционной иммунологии.

125167, Москва

Apollinariya V. Bogolyubova - Cand. Sci. (Biol.), Head of the Laboratory of Translational Immunology.

125167, Moscow

apollinariya.bogolyubova@gmail.comФГБУ «Национальный медицинский исследовательский центр гематологии» Министерства здравоохранения Российской ФедерацииРоссияNational Medical Research Center for HematologyRussian Federation202510072025702208228Copyright © Сердюк А.И., Иванова Н.О., Алешина О.А., Дианов Д.В., Кузнецова В.С., Мохаммад А., Боголюбова А.В., 20252025Сердюк А.И., Иванова Н.О., Алешина О.А., Дианов Д.В., Кузнецова В.С., Мохаммад А., Боголюбова А.В.Serdyuk A.I., Ivanova N.O., Aleshina O.A., Dianov D.V., Kuznetsova V.S., Mohammad A., Bogolyubova A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.htjournal.ru/jour/article/view/637Введение

Введение. Цикл производства Т-лимфоцитов с химерным антигенным рецептором (chimeric antigen receptor, СAR) включает в себя несколько последовательных стадий, параметры которых могут влиять на эффективность трансгенных клеток: получение клеточного материала больного, выделение целевой популяции Т-лимфоцитов, активация, трансдукция клеток вирусным вектором, несущим CAR конструкт, и экспансия полученных CAR T-клеток с дальнейшим введением больному.

Цель

Цель: рассмотреть влияние каждого из этапов производства CAR T-клеток на показатели эффективности клеточного продукта в in vitro и in vivo экспериментах, а также при клиническом применении.

Основные сведения

Основные сведения. Проведен анализ особенностей производства различных CAR T-клеточных препаратов. Обсуждено, как характеристики производства препарата и структуры химерного антигенного рецептора влияют на противоопухолевую эффективность и профиль безопасности клеточного продукта. Получение CAR T-клеточного продукта — многофакторный процесс, который требует оптимизации параметров и обязан учитывать особенности исходного сырья.

Introduction

Introduction. The production cycle of CAR T-cell product includes several sequential stages, each of which may infl uence the efficiency of transgenic cells: obtaining the patient’s cellular material, isolating the target T-lymphocyte population, activation, transduction of the cells with a viral vector carrying the CAR construct, and expansion of the obtained CAR T-cells with further administration to the patient.

Aim

Aim: to review the impact of each of the production steps on the cell product performance in both in vitro and in vivo experiments, as well as clinical applications.

Main findings

Main findings. The manufacturing characteristics of various CAR T-cell products were analyzed in this review, followed by a discussion on how different manufacturing characteristics and chimeric antigen receptor structures affect the antitumor efficacy and safety profi le of the cell product. The production of a CAR T-cell product is a multifactorial process that requires optimization of parameters and must take into account the characteristics of the initial raw material (T-lymphocytes).

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The authors declare that there are no conflicts of interest present.