bloodjourГематология и трансфузиологияRussian journal of hematology and transfusiology0234-57302411-3042ООО Издательский дом «Практика»10.18821/0234-5730-2017-62-4-197-203bloodjour-671Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESИзменения маркеров активации донорских тромбоцитов при хранении после проведения инактивации патогенов с помощью технологии амотосален и ультрафиолетовое облучение спектраChanges of platelet activation markers in donor platelets concentrates during the storage after the inactivation of pathogens by amotosalen and ultraviolet A radiation technologyhttps://orcid.org/0000-0003-4453-7187АзимоваМ. Х.AzimovaM. Kh.

Азимова Мухайёхон Ходжиевна, врач-трансфузиолог отделения клинической трансфузиологии

125167, г. Москва

Azimova Mukhaytkhon Kh., physician of the Department of clinical Transfusiology

Moscow, 125167

maya-azim@mail.ruhttps://orcid.org/0000-0002-9684-5045ГапоноваТ. В.GaponovaT. V.

125167, г. Москва

Moscow, 125167

ГалстянГ. М.GalstyanG. M.

125167, г. Москва

Moscow, 125167

https://orcid.org/0000-0002-8490-6066ГальцеваИ. В.GaltsevaI. V.

125167, г. Москва

Moscow, 125167

https://orcid.org/0000-0001-5932-0285ДавыдоваЮ. О.DavydovaYu. O.

125167, г. Москва

Moscow, 125167

https://orcid.org/0000-0002-6512-910XКапрановН. М.KapranovN. M.

125167, г. Москва

Moscow, 125167

https://orcid.org/0000-0001-8188-5557СавченкоВ. Г.SavchenkoV. G.

125167, г. Москва

Moscow, 125167

ФГБУ «Национальный медицинский исследовательский центр гематологии» Минздрава РоссииРоссияNational Medical Research Center for HematologyRussian Federation201717102025624197203Copyright © Азимова М.Х., Гапонова Т.В., Галстян Г.М., Гальцева И.В., Давыдова Ю.О., Капранов Н.М., Савченко В.Г., 20252025Азимова М.Х., Гапонова Т.В., Галстян Г.М., Гальцева И.В., Давыдова Ю.О., Капранов Н.М., Савченко В.Г.Azimova M.K., Gaponova T.V., Galstyan G.M., Galtseva I.V., Davydova Y.O., Kapranov N.M., Savchenko V.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.htjournal.ru/jour/article/view/671

Представлены результаты по изучению влияния технологии редукции патогенов амотосален и  ультрафиолетовое облучение спектра А на маркеры активации донорских тромбоцитов – антиген CD62P, связывание с антителом PAC-1 под действием аденозиндифосфата (АДФ) – при суспендировании тромбоцитов в цельной плазме донора и в добавочном растворе SSP+, замещающем 70% донорской плазмы, при хранении до 7 сут. Во всех образцах концентратов тромбоцитов в процессе хранения наблюдалась тенденция к увеличению доли спонтанно активированных тромбоцитов и уменьшению доли тромбо- цитов, способных к специфической активации под действием агониста АДФ. Проведение патогенредукции не повлияло на способность к специфической активации тромбоцитов, но привело к увеличению доли спонтанно активированных тромбоцитов. Замещение плазмы раствором SSP+ привело к увеличению доли спонтанно активированных тромбоцитов, но при длительном хранении (к 7-му дню) способствовало сохранению доли функционально активных тромбоцитов.

Pathogen inactivation treatment of platelet concentrates improves the transfusion safety. The influence of the pathogen inactivation on the platelet activation remained unclear. The aim of the study was to  evaluate the changes of the platelet activation markers in donor platelets concentrates during the storage after the inactivation of pathogens by amotosalen and ultraviolet A. By flow cytometry CD62P (P-Selectin) and activated GPIIb/IIIa surface expression were estimated with a mouse-anti-human IgG1 CD62-PE  antibodies and mouse-anti-human GPIIb/IIIa-FITC (PAC-1) antibodies. In platelet concentrates during the storage the fraction of the spontaneously activated platelets increased and fraction of the platelets capable of specific activation under the action of the ADP agonist decreased. The pathogen reduction did not  affect the ability of platelets to specific activity, but led to an increase in the fraction of the spontaneously activated platelets. Replacement of plasma with a solution SSP+ led to the increase in the proportion of spontaneously activated platelets, but during prolonged storage has contributed to the preservation of the fraction of functionally active platelets.

концентрат тромбоцитовредукция патогеновтехнология амотосален+УФАдобавочный раствор SSP+Р-селектинантиген CD62Pантитела PAC-1platelet concentratereduction of pathogenstechnology amotosalen + UVAplatelet additive solution (pas)pHP-selectinCD62P antigenPAC-1 antibodiesReferencesСавченко В.Г., Гармаева Т.Ц., Куликов С.М., Филатов Ф.П., Судариков А.Б., Михайлова Е.А.Эффективность и безопасность трансфузионной терапии гематологических больных. Терапевтический архив. 2006; 7: 12–8.Savchenko V.G., Garmaeva T.Ts., Kulikov S.M., Filatov F.P., Sudarikov A.B., Mikhaylova E.A. The efficacy and safety of transfusion therapy of hematological diseases. Therapeutic archive. Russian journal (Terapevticheskiy arhiv). 2006; 7: 12–18. (in Russian)Vetlesen A., Mirlashari M.R., Torsheim I.A., Kjeldsen-Kragh J. Platelet activation and residual activation potential during storage of hyperconcentrated platelet products in two different platelet additive solutions. Transfusion. 2005;45(8):1349–55.Vetlesen A., Mirlashari M.R., Torsheim I.A., Kjeldsen-Kragh J. Platelet activation and residual activation potential during storage of hyperconcentrated platelet products in two different platelet additive solutions. Transfusion. 2005;45(8):1349–55.Irsch J., Lin L. Pathogen Inactivation of Platelet and Plasma Blood Components for Transfusion Using the INTERCEPT. Transfus. Med. Hemotherapy.2011; 38 (1): 19–31. doi: 10.1159/000323937.Irsch J., Lin L. Pathogen Inactivation of Platelet and Plasma Blood Components for Transfusion Using the INTERCEPT. Transfus. Med. Hemotherapy.2011; 38 (1): 19–31. doi: 10.1159/000323937.Hechler B., Ohlmann Ph., Chafey Ph., Ravanat C., Eckly A., Maurer E., et al. Preserved functional and biochemical characteristics of platelet components prepared with amotosalen and ultraviolet A for pathogen inactivation. Transfusion. 2013; 53(6): 1187–200. doi: 10.1111/j.1537-2995.2012.03923.x.Hechler B., Ohlmann Ph., Chafey Ph., Ravanat C., Eckly A., Maurer E., et al. Preserved functional and biochemical characteristics of platelet components prepared with amotosalen and ultraviolet A for pathogen inactivation. Transfusion. 2013; 53(6): 1187–200. doi: 10.1111/j.1537-2995.2012.03923.x.Bashir S., Cookson Ph., Wiltshire M., Hawkins L., Sonoda L., Thomas S., et el. Pathogen inactivation of platelets using ultraviolet C light: effect on in vitro function and recovery and survival of platelets. Transfusion. 2013; 53(5): 990–1000.Bashir S., Cookson Ph., Wiltshire M., Hawkins L., Sonoda L., Thomas S., et el. Pathogen inactivation of platelets using ultraviolet C light: effect on in vitro function and recovery and survival of platelets. Transfusion. 2013; 53(5): 990–1000.Sandgren P., Diedrich B. Pathogen inactivation of double-dose buffy-coat platelet concentrates photochemically treated with amotosalen and UVA light: preservation of in vitro function. Vox Sang. 2015; 108(4): 340–9. doi: 10.1111/vox.12232.Sandgren P., Diedrich B. Pathogen inactivation of double-dose buffy-coat platelet concentrates photochemically treated with amotosalen and UVA light: preservation of in vitro function. Vox Sang. 2015; 108(4): 340–9. doi: 10.1111/vox.12232.Dumont L.J., Larry J., Jose A., Friedman K.D., Vassallo R.R., Whitley P.H., et al. In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution. Transfusion. 2013; 53(5): 972–80.Dumont L.J., Larry J., Jose A., Friedman K.D., Vassallo R.R., Whitley P.H., et al. In vitro and in vivo quality of leukoreduced apheresis platelets stored in a new platelet additive solution. Transfusion. 2013; 53(5): 972–80.Council of Europe. Guide to the preparation, use and Quality – Assurance of Blood Components. Strasbourg: Council of Europe Press; 2013. Available at: www.edqm.eu.Council of Europe. Guide to the preparation, use and Quality – Assurance of Blood Components. Strasbourg: Council of Europe Press; 2013. Available at: www.edqm.eu.Gulliksson H., AuBuchon P., Cardigan R., Van Der Meer F., Murphy S., Prowse C. Storage of platelets in additive solutions: a multicentre study of the in vitro effects of potassium and magnesium. Vox Sang. 2003; 85(3): 199–205. doi: 10.1046/j.1423-0410.2003.00356.x.Gulliksson H., AuBuchon P., Cardigan R., Van Der Meer F., Murphy S., Prowse C. Storage of platelets in additive solutions: a multicentre study of the in vitro effects of potassium and magnesium. Vox Sang. 2003; 85(3): 199–205. doi: 10.1046/j.1423-0410.2003.00356.x.Gupta A., ChandraT., Kumar A. In vitro function of random donor platelets stored for 7 days in composol platelet additive solution. Asian J. Transfus. Sci. 2011; 5(1): 11–4. doi:10.4103/0973-6247.7569.Gupta A., ChandraT., Kumar A. In vitro function of random donor platelets stored for 7 days in composol platelet additive solution. Asian J. Transfus. Sci. 2011; 5(1): 11–4. doi:10.4103/0973-6247.7569.Азимова М.Х., Галстян Г.М., Гапонова Т.В., Карякин А.В., Крюкова Г.Н., Нехаевская С.С. и др. Биохимические параметры концентратов донорских тромбоцитов при хранении после проведения инактивации патогенов с помощью технологии амотосален + ультрафиолетовое облучение спектра А in vitro. Гематология и трансфузиология. 2017; 62(1): 37–40.Azimova M.Kh., Galstyan G.M., Gaponova T.V., Karyakin A.V., Kryukova G.N., Nekhaevskaya S.S., et al.. Biochemical parameters of donor platelet concentrates during the storage after pathogen inactivation with the aid of technology “Amotosalen + ultra-violet A irradiation” in vitro. Hematology and Transfusiology, Russian Journal (Gematologiya i transfusiologiya). 2017; 62(1): 37–40. (in Russian)Постановление Правительства РФ от 26.01.10 №29 «Об утверждении технического регламента о требованиях безопасности крови, ее продуктов, кровезамещающих растворов и технических средств, используемых в трансфузионно-инфузионной терапии» (с изменениями и дополнениями от 12.10.2010, 4.09.2012). Available at: http://www.consultant.ru/ document/cons_doc_LAW_96793/.Decree of the Government of the Russian Federation No 29 of 26 Jan 2010. On approving the technical regulation about safety requirements of blood, its products, blood-substituting solutions and technical means used in transfusioninfusion therapy. (amendments and supplements of 12.10.2010, 4.09.2012). Available at: http://www.consultant.ru/document/cons_doc_LAW_96793/. (accessed 11 Sept 2017) (in Russian)INTERCEPT viruses inactivation claims. Available at: http://www.INTERSEPTbloodsystem.com/resource-center/technicalda-sheets/INTERCEPTblood-system-for-platelets/INTERCEPTviruses.html; http://www.cerus.com; http://www.амотосален+УФА.bloodsystem.com (accessed 11 Sept 2017)INTERCEPT viruses inactivation claims. Available at: http://www.INTERSEPTbloodsystem.com/resource-center/technicalda-sheets/INTERCEPTblood-system-for-platelets/INTERCEPTviruses.html; http://www.cerus.com; http://www.амотосален+УФА.bloodsystem.com (accessed 11 Sept 2017)Shattil S.J., Hoxie J.A., Cunningham M., Brass L.F. Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. Biol. Chem. 1985; 260(20): 11107–14.Shattil S.J., Hoxie J.A., Cunningham M., Brass L.F. Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. Biol. Chem. 1985; 260(20): 11107–14.Seghatchian J. Platelet storage lesion: an update on the impact of various leukoreduction processes on the biological response modifiers. Transfus. Apher. Sci. 2006; 34(1):125–30. doi: 10.1016/j.transci.2005.09.002Seghatchian J. Platelet storage lesion: an update on the impact of various leukoreduction processes on the biological response modifiers. Transfus. Apher. Sci. 2006; 34(1):125–30. doi: 10.1016/j.transci.2005.09.002Klinger M.H. The storage lesion of platelets: ultrastructural and functional aspects. Ann. Hematol. 1996; 73(3): 103–12.Klinger M.H. The storage lesion of platelets: ultrastructural and functional aspects. Ann. Hematol. 1996; 73(3): 103–12.Rinder H.M., Murphy M., Mitchell J.G., Stocks J., Ault K.A., Hillman R.S. Progressive platelet activation with storage: evidence for shortened survival of activated platelets after transfusion. Transfusion. 1991; 31(5): 409–14.Rinder H.M., Murphy M., Mitchell J.G., Stocks J., Ault K.A., Hillman R.S. Progressive platelet activation with storage: evidence for shortened survival of activated platelets after transfusion. Transfusion. 1991; 31(5): 409–14.Johnston G.I., Bliss B.A., Newman P.J., McEver P.J. Structure of the human gene encoding granule membrane protein-140, a member of the selectin family of the adhesion receptors for leukocytes. J. Biol.Chem. 1990; 265(34): 21381–85.Johnston G.I., Bliss B.A., Newman P.J., McEver P.J. Structure of the human gene encoding granule membrane protein-140, a member of the selectin family of the adhesion receptors for leukocytes. J. Biol.Chem. 1990; 265(34): 21381–85.Abonnenc M., Sonego G., Crettaz D., Aliotta A., Prudent M., Tissot J.D., Lion N. In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVBtreated platelet concentrates. Transfusion. 2015; 55(9): 2219–30. doi: 10.1111/ trf.13123.Abonnenc M., Sonego G., Crettaz D., Aliotta A., Prudent M., Tissot J.D., Lion N. In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVBtreated platelet concentrates. Transfusion. 2015; 55(9): 2219–30. doi: 10.1111/ trf.13123.Rasongles P., Angelini-Tibert M.F., Simon P., Currie C., Isola H., Kientz D., et al. Transfusion of platelet components prepared with photochemical pathogen inactivation treatment during a chikungunya virus epidemic in Ile de La Reunion. Transfusion. 2009; 49(6): 1083–91.Rasongles P., Angelini-Tibert M.F., Simon P., Currie C., Isola H., Kientz D., et al. Transfusion of platelet components prepared with photochemical pathogen inactivation treatment during a chikungunya virus epidemic in Ile de La Reunion. Transfusion. 2009; 49(6): 1083–91.Prowse C.V. Component pathogen inactivation: a critical review. Vox Sang. 2013;104 (3): 183–99. doi: 10.1111/j.1423-0410.2012.01662.xProwse C.V. Component pathogen inactivation: a critical review. Vox Sang. 2013;104 (3): 183–99. doi: 10.1111/j.1423-0410.2012.01662.xRingwald J., Zimmermann R., Eckstein R. The new generation of platelet additive solution for storage at 22°С: Development and Current Experience. Transfus. Med. Rev. 2006; 20(2): 158–64. doi: 10.1016/j.tmrv.2005.11.003.Ringwald J., Zimmermann R., Eckstein R. The new generation of platelet additive solution for storage at 22°С: Development and Current Experience. Transfus. Med. Rev. 2006; 20(2): 158–64. doi: 10.1016/j.tmrv.2005.11.003.de Wildt-Eggen J., Schrijver J., Bins M., Gulliksson H. Storage of platelets in additive solutions:effects of magnesium and/or potassium. Transfusion. 2002; 42(1): 76–80.de Wildt-Eggen J., Schrijver J., Bins M., Gulliksson H. Storage of platelets in additive solutions:effects of magnesium and/or potassium. Transfusion. 2002; 42(1): 76–80.Карпова О.В., Ройтман Е.В., Игнатова А.А., Мадзаев С.Р., Румянцев С.А., Плясунова С.А., Трахтман П.Е. Оценка качества тромбоцитного концентрата, заготовленного аферезным методом, с использованием добавочного раствора SSP+. Вопросы гематологии, онкологии и иммунопатологии в педиатрии. 2014; 13(2): 20–4.Karpova O.V., Roitman E.V., Ignatova A.A.., Madzaev S.R., Rumyancev S.A. Plyasunova S.A., Trahtman P.E. Evaluation of the quality of platelet concentrates prepared by apheresis method and stored in additive solution SSP+. Russian Journal Pediatric Hematology/Oncology and Immunopathology (Voprosy gematologii, onkologii i immunopatologii v pediatrii). 2014; 13(2): 20–4. (in Russian)Miyaji R., Sakai M., Urano H., Nakata K., Sakamoto H., Shirahata A. Decreased platelet aggregation of platelet concentrate during storage recovers in the body after transfusion. Transfusion. 2004; 44(6): 891–9.Miyaji R., Sakai M., Urano H., Nakata K., Sakamoto H., Shirahata A. Decreased platelet aggregation of platelet concentrate during storage recovers in the body after transfusion. Transfusion. 2004; 44(6): 891–9.Bikker A., Bouman E., Sebastian S., Korporaal S.J., Urbanus R.T., Fijnheer R., et al. Functional recovery of stored platelets after transfusion. Transfusion. 2016; 56(5): 1030–7. doi: 10.1111/trf.13544.Bikker A., Bouman E., Sebastian S., Korporaal S.J., Urbanus R.T., Fijnheer R., et al. Functional recovery of stored platelets after transfusion. Transfusion. 2016; 56(5): 1030–7. doi: 10.1111/trf.13544.

The authors declare that there are no conflicts of interest present.