PRODUCTION OF PURIFIED FIBRINOGEN CONCENTRATE
https://doi.org/10.35754/0234-5730-2019-64-1-73-78
Abstract
Aim. To present a technique for obtaining fibrinogen concentrate purified from ballast proteins, which can be used in hemostatic drug production.
Materials and methods. The proposed method is based on isolating fibrinogen from a cryoprecipitate solution by its precipitation with a polyethylene glycol solution followed by virus inactivation by a solvent/detergent. Purification from viral inactivation products and ballast proteins is performed by a two-step processing of the obtained fibrinogen concentrate with glycine solution.
Results. The developed laboratory method for isolating fibrinogen has been optimized in terms of main parameters and scaled in pilot production.
Conclusion. The presented laboratory technique for fibrinogen extraction is characterized by a high yield of the target product, thus being suitable for the production of hemostatic drugs.
About the Authors
V. V. Khurdin
MIREA-Russian Technological University
Russian Federation
Vyacheslav V. Khurdin*, Postgraduate Researcher, Department of Biotechnology and Industrial Pharmacy
Russian Federation
Vyacheslav V. Khurdin*, Postgraduate Researcher, Department of Biotechnology and Industrial Pharmacy
A. L. Berkovskiy
National Medical Research Center for Hematology
Russian Federation
Aron L. Berkovsky, Cand. Sci. (Med.), Consultant
Russian Federation
Aron L. Berkovsky, Cand. Sci. (Med.), Consultant
E. V. Sergeeva
NPO Renam “Society of Hemophilia Patients”
Russian Federation
Elena V. Sergeeva, Production Manager
Russian Federation
Elena V. Sergeeva, Production Manager
A. V. Suvorov
NPO Renam “Society of Hemophilia Patients”
Russian Federation
Alexander V. Suvorov, Cand. Sci. (Biol.), Senior Researcher
Russian Federation
Alexander V. Suvorov, Cand. Sci. (Biol.), Senior Researcher
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Review
For citations:
Khurdin V.V., Berkovskiy A.L., Sergeeva E.V., Suvorov A.V. PRODUCTION OF PURIFIED FIBRINOGEN CONCENTRATE. Russian journal of hematology and transfusiology. 2019;64(1):73-78. (In Russ.) https://doi.org/10.35754/0234-5730-2019-64-1-73-78
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