Clinical, hematological and molecular-genetic features of acute myeloid leukemia with mutations in FLT3, CKIT, NRAS and NPM1

Current methods of the laboratory diagnostics permit to detect a large quantity of the molecular markers, typical for patients with acute myeloid leukemia. However, low frequency of some aberrations does not not to determine their prognostic value. Thus, the necessity of the selection of the most frequent and prognostically significant molecular markers specifies the actuality of the present research. We analyzed the incidence and prognostic relevance of NRAS, CKIT, FLT3 and NPM1 mutations in 200 AML patients. Cytogenetic and molecular-genetic analysis was carried out by GTG-method, PCR and sequencing. We found out, that mutations in CKIT, FLT3 and NPM1 significantly influence on the prognosis, thereby the algorithm of genetic diagnostics of AML patients was suggested. We underlined the importance of the detection of simultaneous mutations in genes with different functionality.

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