Storage of pathogen-reduced platelets

Introduction. To improve the infectious safety of blood products, methods of pathogen inactivation in the donor container are used. Two technologies for pathogen inactivation in platelet concentrates have been approved in Russia: Intercept (amotosalen and ultraviolet A) and Mirasol (ribofl avin and ultraviolet).

Aim — to study the quality parameters of stored pathogen-reduced platelet concentrates.

Materials and methods. The platelet concentrate obtained by automatic plateletpheresis and resuspended in plasma was subjected to pathogen inactivation (PI), and samples were taken for research on the first day of storage before and after PI, as well as on the third and fifth days of storage. The volume of the dose and the number of cells were determined in 60 samples: 30 each, which underwent PI using the Intercept and Mirasol methods. The study of the pH level was performed in 46 platelet concentrates treated with Intercept and 38 containers treated with Mirasol. The characteristics of donors were also taken into account: gender, age, height, weight, and number of donations. The results were evaluated by descriptive statistics and analysis at a significance level of p < 0.05.

Results. In the compared groups, there were no differences in the gender of donors, their donor experience, and anthropometric and hematological parameters. The number of platelets within 3 days in both groups did not differ both from the initial one and between the groups. On day 5, the number of Intercept platelets remained unchanged, and the number of Mirasol platelets significantly decreased both compared with the baseline (425.2 ± 31.0 × 10⁹  vs. 519.6 ± 31.2 × 10⁹, respectively, p < 0.001) and relative to Intercept platelets (425.2 ± 31.0 × 10⁹ vs. 501.9 ± 32.8 × 10⁹, respectively, p < 0.001). During the storage of platelet concentrates, the internal environment of the containers changed to the acidic side, and on day 5, the pH of Mirasol platelets was significantly more acidic than in the comparison group (6.51 ± 0.15 vs. 6.93 ± 0.15, respectively, р < 0.05).

Conclusion. It was found that on day 5 of storage of Mirasol-platelets, the number of cells and pH significantly decreased compared to Intersept-platelets.

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