Влияние различных характеристик клеточного продукта на эффективность анти-CD19 терапии Т-лимфоцитами, модифицированными химерным антигенным рецептором

Введение. Цикл производства Т-лимфоцитов с химерным антигенным рецептором (chimeric antigen receptor, СAR) включает в себя несколько последовательных стадий, параметры которых могут влиять на эффективность трансгенных клеток: получение клеточного материала больного, выделение целевой популяции Т-лимфоцитов, активация, трансдукция клеток вирусным вектором, несущим CAR конструкт, и экспансия полученных CAR T-клеток с дальнейшим введением больному.

Цель: рассмотреть влияние каждого из этапов производства CAR T-клеток на показатели эффективности клеточного продукта в in vitro и in vivo экспериментах, а также при клиническом применении.

Основные сведения. Проведен анализ особенностей производства различных CAR T-клеточных препаратов. Обсуждено, как характеристики производства препарата и структуры химерного антигенного рецептора влияют на противоопухолевую эффективность и профиль безопасности клеточного продукта. Получение CAR T-клеточного продукта — многофакторный процесс, который требует оптимизации параметров и обязан учитывать особенности исходного сырья.

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