Features of immunohematological and hematological parameters of a donor with a rare phenotype -D-
https://doi.org/10.35754/02345730-2020-65-1-52-60
Abstract
Background. This study is devoted to a rare variation of the -D- phenotype . The -D- phenotype was first discovered by R. Race, R. Sanger and J.G. Selwyn in 1951. In Russia, the phenotype -D- was first discovered by V. Morokov in 1985. Typically, the -D- phenotype is detected when physicians examine post-transfusion complications or hemolytic disease of the newborn, since such patients demonstrate high antibody titres to absent antigens. In the present study, the -D- phenotype was detected in a primary blood donor at the clinical laboratory of the Ulyanovsk Regional Blood Transfusion Station (Ulyanovsk, Russia). Aim. To study specific features of immunohematological and hematological blood parameters in a donor with a rare variation of the -D- phenotype.
Materials and methods. The detection of the -D- phenotype by immunohematological methods was carried out using automatic analysers. Molecular DNA typing was used to confirm the -D- phenotype. The shape of erythrocytes of the donor with the -D- phenotype was evaluated using an atomic force microscope. The characteristics of the erythroid lineage were studied using an automatic hematological analyser.
Results. The -D- phenotype was detected in a primary blood donor. Due to the extreme rarity of the -D- phenotype and the lack of programmed algorithms, the validation of the results by automatic analysers was incorrect. Of critical importance was the visual assessment of gel ID cards by the medical staff. Genotyping confirmed the lack of C, c, E, e, Cw specificities in the RHCE gene. The hematological parameters of the donor were within the age norm. An assessment of the image of a cytological blood preparation did not reveal changes in the shape of erythrocytes and their size.
Conclusions. The primary determination of the -D- phenotype using automatic immunohematological analysers can be complicated by the impossibility of validating the results, the incorrect operation of the installed software and the need for expert evaluation of blood samples by the staff. The presented case of the -D- phenotype was not associated with changes in the shape of erythrocytes and blood hematological parameters.
About the Authors
I. M. Lamzin
Ulyanovsk Regional Blood Transfusion Station
Russian Federation
Russian Federation
Ivan M. Lamzin – Cand. Sci. (Med.), Head of Blood Component Production Department.
432017, Ulyanovsk
M. N. Sokolova
Ulyanovsk regional blood transfusion station
Russian Federation
Russian Federation
Marina N. Sokolova – Pathologist the Highest Qualification Category, Head of the Clinical Laboratory.
432017, Ulyanovsk
R. M. Khayrullin
Ulyanovsk State University
Russian Federation
Russian Federation
Radik M. Khairullin – Dr. Sci. (Med.), Prof., Head of the Department of Human Anatomy.
432017, Ulyanovsk
N. V. Mineeva
Russian Research Institute of Hematology and Transfusiology
Russian Federation
Russian Federation
Natalya V. Mineeva – Dr. Sci. (Biol.), Prof., Head of the Isoserology Laboratory.
191024, St. Petersburg
M. E. Khapman
Ulyanovsk regional blood transfusion station
Russian Federation
Russian Federation
Marat E. Khapman – Сand. Sci. (Med.), Chief Doctor.
432017, Ulyanovsk
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Review
For citations:
Lamzin I.M., Sokolova M.N., Khayrullin R.M., Mineeva N.V., Khapman M.E. Features of immunohematological and hematological parameters of a donor with a rare phenotype -D-. Russian journal of hematology and transfusiology. 2020;65(1):52-60. (In Russ.) https://doi.org/10.35754/02345730-2020-65-1-52-60
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