Formation of a cohort of anticovid plasma donors with high-titer antibodies neutralizing SARS-CoV-2
I. B. Simarova,
A. I. Kostin,
Ju. V. Smirnova,
E. A. Ladygina,
D. Yu. Logunov,
I. V. Dolzhikova,
V. V. Ganchin,
E. A. Vasin,
A. I. Baykov,
A. Yu. Bulanov,
N. V. Borovkova,
S. S. Petrikov https://doi.org/10.35754/0234-5730-2020-65-3-242-250
Abstract
Background. The application of convalescent plasma (CP) is currently seen as a feasible therapeutic approach in the treatment of COVID-19.
Aim. To analyze the experience of recruiting a donor cohort from COVID-19 convalescents for banking of CP as part of a pilot project at the Moscow Healthcare Department.
Materials and methods. A retrospective research included 493 COVID-19 convalescents as potential CP donors, all examined at the Sklifosovsky Research Institute for Emergency Medicine. CP was banked using the plasmapheresis method. Only those donors with a documented medical history of COVID-19, which was confi rmed by polymerase chain reaction of SARS-CoV-2 RNA in pharyngeal swabs, and no sooner than 14 days after complete recovery were eligible for donation. Viral neutralizing activity (VNA) was chosen as the key characteristic of the immunological viability of CP. All the donors having VNA titers were characterized in terms of gender, age, time interval since the disease onset, regression of clinical symptoms and clinical features of the COVID 19 course.
Results. Effective (1:160 or more) and acceptable (1:80) VNA titers were found in 21.1 % and 24.75 % of donors, respectively. Signifi cant predictors for a donor having a high VNA titer included: male sex, age over 36 years and verifi ed viral pneumonia. The absence of a signifi cant body temperature response (38.5 °С) can be considered as a negative marker of a potential donor.
About the Authors
I. B. Simarova
N.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Department of Healthcare; City Hospital No. 52 of the Moscow Healthcare Department
Russian Federation
Russian Federation
Iriina B. Simarova, Anesthesiologist-Resuscitator, transfusiologist
A. I. Kostin
N.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Department of Healthcare
Russian Federation
Russian Federation
Alexander I. Kostin, Cand. Sci. (Med.), Head of Transfusion Medicine Department
Ju. V. Smirnova
N.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Department of Healthcare
Russian Federation
Russian Federation
Juliya V. Smirnova, Transfusiologist of the Department of Manufacturing, Clinical Transfusionology and Gravitational Blood Surgery
E. A. Ladygina
N.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Department of Healthcare
Russian Federation
Russian Federation
Elena A. Ladygina, Hematologist, Transfusiologist of the Department of Manufacturing, Clinical Transfusionology and Gravitational Blood Surgery
D. Yu. Logunov
National Research Center for Epidemiology and Microbiology named after N.F. Gamaleya of the Ministry of Health of the Russian Federation
Russian Federation
Russian Federation
Denis Yu. Logunov, Dr. Sci. (Biol.), Corresponding Member of the RAS, Deputy Director
I. V. Dolzhikova
National Research Center for Epidemiology and Microbiology named after N.F. Gamaleya of the Ministry of Health of the Russian Federation
Russian Federation
Inna V. Dolzhikova, Cand. Sci. (Biol.), Head of the Laboratory
Russian Federation
Inna V. Dolzhikova, Cand. Sci. (Biol.), Head of the Laboratory
V. V. Ganchin
Center of Analytical Development of the Social Sector
Russian Federation
Vladimir V. Ganchin, Deputy CEO, Director of the Depar tment of Primary Heal thcare Development
Russian Federation
Vladimir V. Ganchin, Deputy CEO, Director of the Depar tment of Primary Heal thcare Development
E. A. Vasin
City Hospital No. 52 of the Moscow Healthcare Department
Russian Federation
Eugeniy A. Vasin, Analyst
Russian Federation
Eugeniy A. Vasin, Analyst
A. I. Baykov
Center of Analytical Development of the Social Sector
Russian Federation
Alexander I. Baykov, Manager
Russian Federation
Alexander I. Baykov, Manager
A. Yu. Bulanov
City Hospital No. 52 of the Moscow Healthcare Department
Russian Federation
Andrey Yu. Bulanov, Dr. Sci. (Med.)
Russian Federation
Andrey Yu. Bulanov, Dr. Sci. (Med.)
N. V. Borovkova
N.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Department of Healthcare,
Russian Federation
Nataliya V. Borovkova, Dr. Sci. (Med.), Head of the Department of Biotechnology and Transfusionology
Russian Federation
Nataliya V. Borovkova, Dr. Sci. (Med.), Head of the Department of Biotechnology and Transfusionology
S. S. Petrikov
N.V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Department of Healthcare
Russian Federation
Russian Federation
Sergey S. Petrikov, Dr. Sci. (Med.), corresponding member of the RAS
Review
For citations:
Simarova I.B., Kostin A.I., Smirnova J.V., Ladygina E.A., Logunov D.Yu., Dolzhikova I.V., Ganchin V.V., Vasin E.A., Baykov A.I., Bulanov A.Yu., Borovkova N.V., Petrikov S.S. Formation of a cohort of anticovid plasma donors with high-titer antibodies neutralizing SARS-CoV-2. Russian journal of hematology and transfusiology. 2020;65(3):242-250. (In Russ.) https://doi.org/10.35754/0234-5730-2020-65-3-242-250
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